Automated grid freezing system for Cryo-EM chameleon
For high-quality, reproducible CryoEM grid preparation!
We assist in the creation of high-quality Cryo-EM samples. Thanks to the nanowires on the grid surface, blotting of the samples is unnecessary. ● High-speed sample processing without blotting Using hygroscopic grids treated with nanowires allows for rapid sample dispensing in the air. This reduces the effects of the gas-liquid interface and may address issues related to preferred orientation, aggregation, or denaturation. ● Automated grid processing A series of operations from sample application to freezing of the grid set in the grid holder is automated. The movements are precisely controlled, preventing manual damage or loss. ● Sample tracking and recording To ensure reproducibility, relevant parameters and grid images are recorded. ● Features ◻︎ Glow discharge device integrated into the workflow inside the main unit ◻︎ Function to adjust the drop speed of the tweezers ◻︎ QC system after sample dispensing ◻︎ Data tracking and reporting capabilities
basic information
< chameleon > ■ Minimum sample dispensing volume: 6 nL ■ Minimum sample preparation volume: 5 μL ■ Standard time required from dispensing to freezing: 101 milliseconds ■ Shortest time required from dispensing to freezing: 54 milliseconds ■ Sample block temperature range: above 4℃, below 37℃ ■ Power supply: Single-phase 115/230V (+/-10%) 50/60Hz ≦6A ■ Weight: 225kg ■ Main unit size (mm): W916 x D708 x H1687
Price information
Please contact us.
Delivery Time
Model number/Brand name
chameleon
Applications/Examples of results
Chameleon is a product developed from the system previously researched and developed as "Spotiton." The core idea was conceived by the group of Bridget Carragher and Clint Potter at the New York Structural Biology Centre (NYSBC). They focused on the limitations of existing grid preparation methods and aimed to develop a more reliable and reproducible technique for grid vitrification by miniaturizing sample dispensing using inkjet technology. Spotiton, published in 2012, has undergone numerous improvements and contributed to an increase in cutting-edge publications on high-resolution structural analysis and interfacial effects. Chameleon leverages the advantages of Spotiton and incorporates the automation technologies that SPT Labtech has cultivated in the field of structural biology. For the latest information, please contact SPT Labtech Japan.
