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Supercritical fluid chromatography-mass spectrometry method

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Supercritical fluid chromatography-mass spectrometry method

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Supercritical fluid chromatography is a method that uses supercritical CO2 as the mobile phase to separate components contained in a sample and detect them with a mass detector.

■What is Supercritical Fluid? A supercritical fluid is a state that exists under conditions of temperature and pressure above the critical point, possessing properties that are intermediate between those of a liquid and a gas (see Figure 1). Supercritical CO2 can be used as a mobile phase in chromatography due to its ease of handling at conditions (7.4 MPa, 31°C). ■Extraction Using Supercritical CO2 In the SFC/MS setup, SFE (Supercritical Fluid Extraction) is connected in the preceding stage, and extraction of components contained in the sample is performed using supercritical CO2. Since the extraction, separation, and detection can be carried out automatically, it is effective for the analysis of components that are sensitive to light, water, and oxygen.

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Supercritical Fluid Chromatography_B0309

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