Application Note: Rapid Separation of Cholesterol by ELSD
UV & ELSD double trigger fractionation system Pure C-815! Highly productive cholesterol ELSD rapid fractionation.
Related Device: Fractionation Chromatography System Pure C-815 Cholesterol and its esters, which are components of biological membranes, are utilized as industrial raw materials in cosmetics, pharmaceuticals, and liquid crystals. When performing gradient fractionation purification for compounds that are difficult to detect with UV detectors, methods are selected using low wavelength UV detection such as 210 nm or ELSD (Evaporative Light Scattering Detector). ELSD is a fractionation purification method that overcomes the disadvantages of UV-triggered fractionation due to the absence of baseline drift and T0 peaks. By adopting ELSD & UV double triggering, it enables complementary fractionation purification using both ELSD and UV. 【Issues with Low Wavelength UV Triggered Fractionation】 ■ The baseline drifts due to the solvent's own absorption used in the mobile phase, making it difficult to detect sample peaks. ■ Baseline rises consistently during gradient steps, leading to the baseline being recognized as a peak, which significantly increases purification time and fraction volume. *For more details, please refer to the PDF document or feel free to contact us.
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【Product Specifications of Pure C-815 (Partial)】 ■Flow Rate (mL/min): 250 ■Maximum Pressure (MPa): 5.0 ■Number of Solvent Channels: 4 ■UV Spectrum (nm): 200-800 ■UV Fractionation Trigger: 4 Wavelengths ■UV Scanning Function: Standard ■Safety Features: 4 Sensors Standard Equipped ■Dimensions (W×D×Hmm): 365×570×680 *For more details, please refer to the PDF document or feel free to contact us.
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For more details, please refer to the PDF document or feel free to contact us.
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Since 1957, when Büchi was the first in the world to commercialize rotary evaporators, we have been leading the world in high-precision analytical support technologies. In recent years, we have steadily expanded our achievements in the fields of inspection and component analysis of pharmaceutical and food raw materials using near-infrared analysis. At Büchi Japan, we always prioritize our customers and support their businesses by providing high-quality products, systems, solutions, applications, and services that deliver added value.