Quantitative three-dimensional culture colony assay kit
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Professor Tatsuhiko Furukawa, Molecular Oncology, Graduate School of Medical and Dental Sciences, Kagoshima University, Collaborative Research and Development.
■Features ● Possesses anchorage-independent proliferation ability of cancer cells, applicable to anticancer drug development and cancer research through colony formation assays. ● Higher accuracy and lower variability in analysis of multiple samples compared to conventional colony methods. ● Streamlined into a product set for quick and easy use, requiring no special reagents (operation time reduced to less than half). ● Cost reduced to about 1/10 of conventional products.
basic information
A research paper using the "Quantitative Three-Dimensional Culture Colony Assay Kit," in collaboration with Professor Satoru Koyanagi from the Graduate School of Pharmaceutical Sciences at Kyushu University, has been published in the American scientific journal Journal of Biological Chemistry. Chiharu Katamune, Satoru Koyanagi, et al. [http://www.jbc.org/content/early/2016/03/09/jbc.M115.706481.abstract] Different roles of negative and positive components of the circadian clock in oncogene-induced neoplastic transformation JBC Papers in Press. Published on March 9, 2016 as Manuscript M115.706481 In this study, the "evaluation of cell carcinogenesis" using the assay kit was a key point, and we were able to repeatedly obtain simple and stable experimental results with the assay kit.
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Applications/Examples of results
● Cancer drug discovery screening ● Drug metabolism enzyme induction evaluation system ● Optimization of culture conditions for primary cancer cells ● Optimization of proliferation conditions for cancer stem cells
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| Model number | overview |
|---|---|
| CAK-RPMI-8 | |
| CAK-DMEM-8 |
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Since its establishment in 1957, Nippon Medical Instrument Manufacturing Co., Ltd. has steadily grown as a partner for researchers and doctors. We have supplied products to various government agencies, numerous private research institutions, educational institutions including national, public, and private universities, and medical institutions such as university hospitals, while also engaging in the design and construction of many important facilities. Being involved in cutting-edge fields and applying our latest technologies and know-how is a great encouragement and joy for us as a manufacturer. In 2002, we obtained ISO 9001 certification for our quality management system, ensuring that all processes from planning and development to manufacturing, sales, and after-sales service are conducted with a robust system in place. As we enter the 21st century, the global situation and economy are becoming increasingly complex, but we are committed to addressing corporate activities swiftly and resiliently, with a correct perspective on the past, present, and future for the rich progress of the Earth and humanity.