Cytiva Official site

Tangential flow filtration (TFF) is a filtration method that applies a constant pressure to the membrane side by flowing liquid samples parallel to the membrane surface, allowing solutes and small molecules to pass through the membrane. Compared to conventional direct flow filtration, TFF is less prone to filter clogging because it is continuously perfused parallel to the membrane surface, enabling efficient and large-capacity ultrafiltration. The Minimate EVO TFF system is suitable for large-scale protein and nucleic acid purification and is equipped with Omega membranes that have a wide range of molecular weight cut-offs (MWCO) from 1K to 1000K. It is also suitable for applications that require the purification of exosomes, which are extracellular nano-vesicles, from large volumes of cell culture media. It has been reported that TFF methods have a higher recovery rate of exosomes from cell culture media compared to ultracentrifugation [1], and there are also proven results with Minimate [2]. If you are considering large-volume sample ultrafiltration, please feel free to contact us. [1] Busatto S., et al., Cells, 7, 273-283 [2] Sung SE., et al., Int J Mol Sci, 22, 2889-2900

The Minimate EVO system is a large-capacity sample (~1 L) ultrafiltration system by TFF. Demonstrations and rentals are available! Try it with actual samples! 【Features】 - Fast and efficient: Concentration and desalting operations can be performed simultaneously. - High recovery rate: Achieves a high recovery rate with low protein adsorption. - Economical: Depending on the application, membranes can be cleaned and reused. - Compact: Compact size takes up little space. 【Package Contents】 • MasterFlex® L/S variable speed pump, with L/S Easy-Load pump head • VWR® magnetic stirrer • 500 mL sample reservoir with lid (includes fittings and O-rings for the lid) • Fittings and O-rings • Drip tray • Pressure gauge • Adjustable drip tray legs • Power cord for the pump (100V-250V AC: not a current conversion plug) • Power cord for the magnetic stirrer (power 100V-240V AC, input voltage 12V DC) • Fittings/parts bag

For inquiries regarding delivery dates, prices, and demo requests, please use the inquiry form or the contact button.

Minimate EVO TFF System

● Concentration and desalting of proteins, peptides, and nucleic acids (DNA, RNA, oligonucleotides) ● Purification and concentration of exosomes ● Recovery of antibodies and recombinant proteins from culture supernatants after cell (microbial) separation ● Separation processes for metal-sensitive enzymes and molecules ● Concentration of viruses and gene therapy vectors ● Sample pretreatment before column chromatography ● Sample concentration after gel filtration ● Removal of pyrogens from water, buffer solutions, and culture media solutions