The new application report describes how to rescue low-expressing cells using fluorescence-activated cell sorting (FACS) and perform efficient APC assays.
ソフィオンバイオサイエンス
The Kv1.3 ion channel regulates the activation of T lymphocytes and controls membrane potential and calcium signaling in immune responses. Dysregulation is associated with autoimmune diseases such as multiple sclerosis, psoriasis, and rheumatoid arthritis. The Kv1.3 channel also plays a role in the proliferation and survival of cancer cells. Therefore, Kv1.3 channels are considered promising targets for the treatment of both autoimmune diseases and cancer. Efficient ion channel assays require consistent, robust, and functional membrane expression. Using a low-expressing Kv1.3 cell line, we classified cells based on Kv1.3 expression using the Tyto cell sorter (from Miltenyi Biotec), significantly improving the success rate of QPatch assays. For more details, please see the related links.
