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Spot indole reagent

Detection of indole production by intestinal bacteria and certain anaerobic bacteria!

The "Spot Indole Reagent" is a product that demonstrates the ability of specific bacteria to decompose the amino acid tryptophan and convert it into indole, accumulating it in the medium. In the spot test, indole binds to p-dimethylaminocinnamaldehyde (DMACA) in an acidic pH within the filter paper matrix, producing a blue to blue-green compound. In a positive result, a color change from blue to blue-green appears in the bacterial smear sample within 2 to 3 minutes. If there is no reaction, it is considered negative. 【Specifications】 ■ Product Number: SG-SPOTIND-5 ■ Volume: 5mL *For more details, please download the PDF or feel free to contact us.

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basic information

【Instructions】 1. Use a sterile swab to collect a portion of the colony cultured for 18 to 24 hours from non-selective media. 2. Drop spot indole onto the collected sterile swab. *For more details, please download the PDF or feel free to contact us.

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