Setting of gradient conditions for ion chromatography
Thorough explanation of how to set gradient conditions! It will provide hints for improving separation.
Generally, isocratic conditions are used for elution in ion chromatography; however, when analyzing diverse ionic species, separation can be difficult and may not be achievable under isocratic elution conditions. In such cases, using hydroxide ions and performing gradient analysis with a concentration gradient can improve separation. Gradient analysis is a method that enhances separation by increasing the concentration of the eluent from low to high during the analysis, allowing components that are retained more easily to elute faster. We will introduce examples of the separation of inorganic anions and organic acids regarding the separation of ionic species due to changes in eluent concentration and the setting of gradient conditions. By using an eluent generator system, it is possible to supply accurate and high-purity eluent simply by sending ultrapure water. Additionally, gradient analysis can be easily performed with just the concentration settings from the workstation. Furthermore, the Thermo Scientific Virtual Column Separation Simulator can generate virtual chromatograms under gradient conditions online. Please utilize it for examining analytical conditions.
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