[HPLC Case Study] Analysis of Organic Acids in Beverages Using Post-Column Analysis Method

Accurate quantification of organic acids in beverages! Introduction to the performance and convenient features of a post-column HPLC system suitable for routine analysis.

This technical document introduces a post-column analysis system for accurately quantifying organic acids in various beverages. The analysis column uses ion exclusion mode, and by adding BTB solution post-column, it separates and detects ten types of organic acids with good reproducibility. The e-workflow feature, which supports operations in routine analysis, is a significant advantage as it fully automates the process from device startup to measurement and report generation. The HPLC system features a compact design that incorporates a dual pump with two pumps in one module for analysis and post-column solution delivery. Additionally, even in the event of an error, the automatic switching of valves prevents the post-column reaction solution from backflowing into the analysis column, allowing for safe adoption in routine analysis. 【Features】 ■ Accurate quantification of ten types of organic acids with good reproducibility of less than 1% ■ Separation of organic acids using ion exclusion mode columns, allowing measurement without matrix effects ■ Detection limits for organic acids in this method range from a few to 7 μg/mL ■ Automation of the process from device startup to report generation with the e-workflow feature ■ Ensured safety with automatic valve switching in case of errors

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basic information

[Device Configuration] Vanquish Core HPLC System Pump: Thermo Scientific Vanquish Dual Pump C Autosampler: Thermo Scientific Vanquish Split Sampler CT Column Oven: Thermo Scientific Vanquish Column Compartment C (2 units) Detector: Thermo Scientific Vanquish Diode Array Detector C Software: Thermo Scientific Chromeleon Chromatography Data System 7.3