Cell Cryopreservation Solution "Cryoscalers DMSO-Free"
After thawing, it shows a high survival rate in various cultured cells and maintains the pluripotency of stem cells (undifferentiated state).
"Cryoscalis DMSO-Free" is a cell freezing preservation solution that allows for approximately 90% survival rate even after thawing, while maintaining the differentiation potential of stem cells during cryopreservation. It does not contain proteins or DMSO and can be stored stably at 4°C for long periods. Due to the absence of toxicity from DMSO and the influence of proteins, cells can be cryopreserved safely with a high survival rate. 【Features】 ■ Contains no serum or protein components ■ Approximately 90% survival rate even after thawing ■ Stem cells can be cryopreserved while maintaining their differentiation potential ■ Confirmed to be free from bacterial, fungal, and mycoplasma contamination through sterility testing ■ Can be stored stably at 4°C for long periods *For more details, please refer to the related links or feel free to contact us.
basic information
【Overview of Operation Method】 ■ Place cultured cells in a tube, centrifuge, and remove the supernatant. ■ Add this product to suspend the cells to a concentration of 5×10^5 to 5×10^6 cells/ml, and aliquot 1 ml into storage tubes. ■ Freeze by placing in a -80℃ deep freezer. * For long-term storage, keep in liquid nitrogen. ■ Rapidly thaw the stored cells in a 37℃ incubator while shaking, and centrifuge wash with an appropriate medium (about 10 ml). * For more details, please refer to the related links or feel free to contact us.
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Applications/Examples of results
【Usage】 ■ Freezing preservation solution for cells *For more details, please refer to the related links or feel free to contact us.
