Application Note: Automation of Cell-Based Assays
Drug efficacy profiling against anticancer agents using the NCI-60 cell line panel.
The NCI-60 panel is a collection of various cancer cell lines and is one of the most important tools in drug discovery for evaluating the activity of anticancer agents. While cell lines are commonly used in molecular biological assays, when combined with bioinformatics such as the NCI COMPARE database, results obtained from the NCI-60 panel can also estimate the mechanisms of drug efficacy against novel compounds. The efficacy evaluation for a large variety of compounds is initially tested at a single concentration. Hit compounds that show cell growth inhibition or cytotoxicity are then profiled in detail against multiple cell lines by creating dilution series. Tekan and the German biotech company European ScreeningPort GmbH have developed a protocol to demonstrate this cell-based assay solution. By combining expertise in cell-based assays from automated liquid dispensing to detection, they provide fast and reliable fully automated assays. After sample preparation using Fluent, cell viability was measured using luciferase detection for cellular ATP measurement (CellTiter-Glo, Promega).
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398334
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Cell-based assay
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Tecan is a company that was established in Switzerland in 1980, specializing in the manufacturing and development of life science research support equipment. With the support of many customers, we were able to celebrate our 30th anniversary in Japan in 2022. Tecan Japan operates two businesses. One is the life sciences business, which delivers Tecan-branded products such as automated pipetting systems and microplate readers to end-users. The other is the partnering business, which provides components and equipment as OEM products for partner companies. Our customers span a wide range of fields, including disease research, drug discovery, biotechnology, clinical testing (such as cancer diagnosis, infectious disease diagnosis, and testing centers), scientific analysis, and robotic surgery. Our mission is to improve people's lives and health. Tecan Japan aims to contribute to global health care innovation from the life sciences industry to clinics by supporting our customers' daily activities.