[Application Note] Simplifying Complex Protocols with Automatic Dispensers
Automation of H3K27Me3 cell assays with Fluent.
Cisbio EPIgeneous cell-based assays allow for the direct measurement of methylation, demethylation, and total protein in both adherent and suspended cells. Tecan has achieved automation using the Fluent dispensing system, which is built based on the specific needs of lab applications. Fluent provides greater capacity and speed. This platform offers excellent throughput and walkaway time, enabling more reliable results and making it easier to perform many experimental processes. Tecan, in combination with Cisbio, known for its HTRF technology, has developed new protocols by combining expertise in automated liquid handling, detection, and cell-based assays. This enables the execution of fast, reliable, and fully automated assays.
basic information
Epigenetics has played an important role in drug discovery for many years, but its significance has recently increased markedly in understanding the role of epigenetic modifications in cancer and stem cell differentiation. Methylation is a major epigenetic modification of DNA and histones that is involved in the regulation of gene expression. Epigenetic changes may persist throughout the lifespan of the cell itself due to cell division. They may also last across multiple generations, even in the absence of changes to the underlying DNA sequence of the organism. Therefore, epigenetic modifications can potentially be inherited. These modifications can be influenced by environmental factors and may trigger epigenetic effects that are involved in biological processes such as aging.
Price range
Delivery Time
Model number/Brand name
398370
Applications/Examples of results
Cell-based assay
