More efficient evaluation of 3D cell assays.
<Free Offer> Complete one device for spheroid production, culture, real-time monitoring, and endpoint analysis - Technical Note
Three-dimensional cell culture that can reproduce an environment close to that in vivo. Spheroids can be easily created using "round-bottom plates" without scaffolds, but do you feel stressed by issues like "it's difficult to focus the microscope..." or "I want to track changes over time, but I'm concerned about the cost of staining each time and the toxicity to the cells"? With Tecan's microplate reader "Spark Cyto," you can utilize its unique image-based autofocus. It enables a series of assay processes from "label-free long-term growth monitoring" using AI image recognition in bright field to "quantitative evaluation" using fluorescence, all in one device. This technical note provides a detailed explanation of methods for effortless and consistent observation and evaluation of spheroid production using round-bottom microplates with Spark Cyto. Additionally, examples are provided to illustrate how to streamline experimental workflows by combining it with a plate washer. *The main document is the "【Technical Note】Real-time Growth Monitoring of Spheroids in Round-Bottom Microplates," located at the top of the catalog list below.*
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【What You Can Learn from This Document】 ◎ Examples of spheroid production and observation that improve positional and focus shifts unique to round-bottom microplates. ◎ A method for conducting label-free real-time growth monitoring to final quantification using a single microplate reader. ◎ Examples of utilizing the HydroSpeed washer to prevent spheroid suction loss and automate the washing process. 【Equipment Used】 ■ Microplate reader "Spark Cyto" with bright field and fluorescence imaging capabilities. ■ Plate washer "HydroSpeed." *For more details, please refer to the document. Feel free to contact us with any inquiries.
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*For more details, please refer to the materials. Feel free to contact us as well.*
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Tecan is a company that was established in Switzerland in 1980, specializing in the manufacturing and development of life science research support equipment. With the support of many customers, we were able to celebrate our 30th anniversary in Japan in 2022. Tecan Japan operates two businesses. One is the life sciences business, which delivers Tecan-branded products such as automated pipetting systems and microplate readers to end-users. The other is the partnering business, which provides components and equipment as OEM products for partner companies. Our customers span a wide range of fields, including disease research, drug discovery, biotechnology, clinical testing (such as cancer diagnosis, infectious disease diagnosis, and testing centers), scientific analysis, and robotic surgery. Our mission is to improve people's lives and health. Tecan Japan aims to contribute to global health care innovation from the life sciences industry to clinics by supporting our customers' daily activities.