Basics of Ion Chromatography: Temperature Affecting Separation
A clear explanation of the basics of ion chromatography! Let's understand the impact of changes in column temperature on separation.
Ion exchange separation occurs due to the competition between the measurement ions and the eluent ions on the ion exchange sites. One of the factors that affects this ion exchange separation is the change in column temperature. When the column temperature is altered, the retention time of the measurement ions changes due to variations in separation equilibrium, diffusion rates, degree of dissociation, and the viscosity of the eluent. The impact of temperature varies depending on the type of measurement ions and also changes with different columns and eluents. This factor of temperature will be explained.
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