[Case Study] Simultaneous Analysis of Vitamins A, D, and E using 2D-LC

Simplifying the preprocessing with the single loop heart cut 2D-LC method significantly reduces analysis time. It enables the highly sensitive detection of vitamins A, D, E, and their isomers.

This technical document introduces a rapid and quantitative analytical method for the simultaneous detection of seven types of vitamins (vitamin A, vitamin D2, vitamin D3, and four types of vitamin E isomers) in infant formula using a conventional HPLC system with a diode array detector and a fluorescence detector, following offline solid-phase extraction (SPE) and two-dimensional liquid chromatography (2D-LC) analysis. By changing the traditional liquid-liquid extraction method to solid-phase extraction, the time required for sample preparation was reduced from 3 hours to 1 hour. Additionally, by simplifying the sample preparation and optimizing the separation conditions, the influence of the matrix was reduced, allowing for the detection of target substances with higher sensitivity. ■ Simplified sample preparation through two-dimensional liquid chromatography (2D-LC) analysis ■ Simultaneous separation analysis of vitamins A, D, E, and their isomers ■ Developed a method with higher recovery rates and sensitivity than liquid-liquid extraction ■ Simplified the preprocessing process and reduced analysis time by 2.5 times ■ Reduced the influence of the matrix and provided reliable data

For more details about the Thermo Scientific Vanquish online 2D-LC system, click here.

basic information

System Configuration HPLC: Vanquish Core HPLC System Pump: Thermo Scientific Vanquish Dual Pump Auto-sampler: Thermo Scientific Vanquish CT Column Oven: Thermo Scientific Vanquish Column Compartment C Detector: CG Diode Array Detector Thermo Scientific Vanquish DAD (Flow Cell: 13 μL), Fluorescence Detector Thermo Scientific FLD F (Flow Cell: 8 μL) Valves: 2 position 6 port valves (2 units) Software: Thermo Scientific Chromeleon Chromatography Data System 7.3