[Case Study] Analysis of Tocopherols α, β, γ, and δ using 2D-LC
Vitamin E (alpha, beta, gamma, delta tocopherols) in vegetable oils can be separated and quantified without the influence of interfering components using single-loop heart-cut 2D-LC.
This document introduces a method for the high-precision separation and quantification of four types of tocopherols contained in vegetable oils using the single-loop heart-cut 2D-LC method. Under the separation conditions of a reverse-phase system using a general ODS column, the separation of β-tocopherol and γ-tocopherol is difficult; however, the 2D-LC method allows for easy separation. In the first dimension, tocopherols and interfering components in the sample are separated, and in the second dimension, the four types of tocopherols (α, β, γ, δ) are separated. The repeatability of the standard samples achieved a high reproducibility with RSD < 0.1%. Additionally, measurements conducted on multiple vegetable oils showed that quantification was possible without the influence of interfering peaks. The single-loop heart-cut 2D-LC method is the simplest technique among 2D-LC methods, making it applicable for routine analysis and a useful tool for food analysis involving samples with a high content of matrix components. 【Features】 ■ High-precision separation achieved with the single-loop heart-cut 2D-LC method ■ Separation of β-tocopherol and γ-tocopherol, which is difficult in reverse-phase systems ■ Effective separation of interfering substances in food ■ High reproducibility with repeatability of RSD < 0.1%
basic information
Equipment configuration of the Thermo Scientific Vanquish Single Loop Heart Cut 2D-LC System Pump: Thermo Scientific Vanquish Dual Pump F Autosampler: Thermo Scientific Vanquish Split Sampler FT Column thermostat: Thermo Scientific Vanquish Column Compartment H Two diode array detectors: Thermo Scientific Vanquish DAD FG, Thermo Scientific Vanquish DAD HL Valve: 2 position 6 port valve Software: Thermo Scientific Chromeleon Chromatography Data System 7.3
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