[Case Study] Characterization of Lipid Components in LNP Formulations Using LC-CAD

Simplifying sample pretreatment with high-sensitivity CAD, achieving rapid and accurate identification of lipid components and molar ratio evaluation.

The development and quality control of LNP formulations require rapid and accurate identification of lipid components and evaluation of molar ratios. This technical document introduces a method for efficiently quantifying the concentration and molar ratio of each lipid in LNP formulations. The Charged Aerosol Detector (CAD) is a versatile detector for liquid chromatography that can detect all non-volatile and semi-volatile compounds. It is particularly sensitive for detecting compounds that do not absorb UV light, such as LNPs. Furthermore, by leveraging the CAD's high sensitivity, consistent responsiveness, and wide dynamic range, we aim to simplify sample preparation and facilitate method development. Additionally, by using the Thermo Scientific Accucore C30 LC column, we can effectively separate lipid components and other excipients in LNP formulations in a short time. This has also confirmed that the measurement results align well with the theoretical molar ratio%. 【Features】 ■ Rapid and accurate identification of lipid components and evaluation of molar ratios ■ Compliance support with Thermo Scientific Chromeleon CDS software ■ Effective separation with Accucore C30 LC column ■ Measurement results that align well with theoretical molar ratio%

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