[Case Study] High-Precision Profiling Technology of Polysorbate 80
HPLC-CAD and single quadrupole mass spectrometry can detect inter-lot comparisons and quality issues of Polysorbate 80!
This technical document explains an HPLC method suitable for the fingerprinting of Polysorbate 80 and proposes a simple approach to detect variations between different suppliers, grades, and manufacturing lots, as well as to identify quality issues. The Thermo Scientific ISQ EM single quadrupole mass spectrometer, with a mass range up to m/z 2,000, allows for the easy identification of a wide range of key components of polysorbates from low to high molecular weights. Additionally, the consistent response of the CAD and the reverse gradient method enable semi-quantification without standard substances, allowing for a substantial understanding of the mass balance between components with varying degrees of esterification. Please take a look at the optimal HPLC-CAD-MS method for fingerprinting Polysorbate 80. *For more details, please download the PDF or feel free to contact us.*
basic information
【System Configuration】 Thermo Scientific Vanquish Flex Duo UHPLC System - Pump: Thermo Scientific Vanquish Dual Pump F, Vanquish Quaternary Pump F - Autosampler: Thermo Scientific Vanquish Split Sampler FT - Column Oven: Thermo Scientific Vanquish Column Compartment H - Detector: Thermo Scientific Vanquish CAD H - Thermo Scientific Vanquish Duo Back Gradient Kit Thermo Scientific ISQ EM Single Quadrupole Mass Spectrometer Software - Thermo Scientific Chromeleon Chromatography Data System 7.3 - Thermo Scientific FreeStyle 1.6 Software
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Applications/Examples of results
For more details, please download the PDF or feel free to contact us. It can only be used for research purposes and cannot be used for diagnostic purposes.
