[Case Study] Analysis of Isotope-Labeled VOCs Using High-Resolution GC-MS
Identification of labeled components using high-resolution and precise mass measurement with Orbitrap! Introduction to applications in metabolic flux analysis and biomarker discovery.
This application note presents a case of highly selective detection of isotope-labeled VOCs (volatile organic compounds) using the Thermo Scientific Orbitrap Exploris GC mass spectrometer. Using the search for biomarkers related to ferroptosis as an example, VOCs derived from cells labeled with oxygen-18 (¹⁸O) were measured using thermal desorption (TD)-GC-MS. The high-resolution and precise mass measurement demonstrated the ability to distinguish between labeled and unlabeled components, allowing for the separation and identification of co-eluting isobaric ions. Furthermore, analytical methods useful for applications in metabolic flux analysis are introduced, ranging from the estimation of labeled components using Compound Discoverer software to confirmation via precise mass spectra. 【Features】 ■ Sample preparation and TD-GC-MS measurement conditions for ¹⁸O-labeled VOCs are provided. ■ The mass shift of +2.0042 Da due to ¹⁸O labeling is confirmed using 1-Octen-3-ol as an example. ■ Examples of the identification of co-eluting ions around m/z 85 and 87 are presented.
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Thermo Fisher Scientific K.K., the Japanese subsidiary of Thermo Fisher Scientific Incorporated, is a sales company for comprehensive system analysis instruments, bio-related equipment, and research reagents. In Japan, it has bases in Yokohama, Tokyo, Osaka, Fukuoka, and Nagoya, handling various analytical instruments including mass spectrometers, various bio-related equipment, measuring instruments, medical devices, lab software, and research reagents.