**[Challenge]** Even with high-purity samples like cell culture samples, accurately characterizing extracellular vesicles (EVs) is not easy. Interference from lipoproteins, cellular debris, and protein aggregates poses challenges. Additionally, the complexity of biological fluids, limited sample volumes, and the presence of rare EV subpopulations further complicate the analysis. **[Solution]** Our Leprechaun separates EVs on Luni consumables, allowing for the measurement of particle size and concentration, and simultaneous analysis of up to four surface or internal markers. It can be applied to samples of less than 25µL, does not require sample purification, and offers sensitivity up to 5×10⁵ particles/mL, single particle analysis, and EV size measurement down to 35nm. **[Demonstration]** In this webinar, we will introduce how Leprechaun combines single particle interference measurement with immunofluorescence imaging to achieve comprehensive analysis of any EV sample. It is also possible to confirm the loading efficiency of cargo carried by EVs, distinguish whether proteins are soluble or EV-derived, and obtain a breakdown of all EV subpopulations.

Unchained Labs, Inc. will exhibit at the 41st Annual Meeting of the Japanese DDS Society, which will be held from June 17 (Tuesday). At our booth, you can see a wide range of products, from the manufacturing of lipid nanoparticles (LNP) to their characterization, as well as products for ADC and the evaluation of extracellular vesicles. Additionally, on the first day of the event, July 17, we will also hold a presentation by exhibiting companies. All of our staff sincerely look forward to your visit. Event Name: 41st Annual Meeting of the Japanese DDS Society Date: July 17 (Tuesday) - July 18 (Wednesday) Venue: Makuhari Messe International Conference Hall Details: https://procomu.jp/dds2025/ <Exhibiting Company Presentation> July 17 (Tuesday) around 15:28

CPhI Japan Exhibition & Seminar! April 9 (Wednesday) - 11 (Friday) @ Tokyo Big Sight Unchained Labs, Inc. will be exhibiting in the biopharmaceutical zone at CPhI Japan this year as well. Additionally, we will hold a seminar on the second day! Please visit our booth at Tokyo Big Sight. <Exhibitor Seminar> Date and Time: April 10 (Thursday) 13:00-13:30 Venue: 5B "Stability Evaluation of Biopharmaceuticals - Towards a New Era of High-Speed and High-Throughput" *No prior reservation required, free of charge.

<Announcement of Interphex Exhibition and Product Technology Seminar> Our company will be exhibiting at the Interphex Pharma Lab EXPO again this year. Additionally, on the first day of the exhibition, July 5th, we will also be presenting at a product technology seminar! We invite you to come to Tokyo Big Sight. Exhibition Details: Name: Interphex Week Tokyo (25th Edition) Date: July 5 (Wednesday) - July 7 (Friday) 10:00-17:00 Venue: Tokyo Big Sight, East Hall 1, Booth: 1A-10 Details of our exhibited products: https://www.interphex.jp/tokyo/ja-jp/search/2023/directory/directory-details.org-fc5952a0-dbf6-4ef7-bfa0-2feb60a9169f.html Product Technology Seminar: "Innovative Next Steps in Pharmaceutical Development Using Lipid Nanoparticles" We will introduce a solution that allows for high-throughput processes from the examination of conditions for lipid nanoparticle production to buffer exchange and physical property evaluation. Date and Time: July 5 (Wednesday) 12:30-13:00 Venue: Room 4 (East Hall 5)

Challenge: Monitoring the titer, structural stability, and non-viral contaminants of lentiviruses during production is crucial for generating high-yield and high-purity pharmaceuticals. However, traditional methods such as ELISA and qPCR require sample purification and cannot perform structural analysis of the virus, making it very difficult to obtain accurate viral titers that are not affected by fragmented viral particles or contaminants. Solution: Unchained Labs' new product, Leprechaun, is a platform that provides critical information about crude or purified lentivirus samples. Leprechaun measures particle size to separate individual lentiviruses from aggregates and uses fluorescent antibodies to check for the presence of VSVG, capsid, soluble p24, and contaminants from extracellular vesicles (EVs). By using Leprechaun, it is possible to monitor titer and purity throughout the entire workflow of lentivirus research and development. In this webinar, we will explain how Leprechaun reveals the truths about your lentivirus that you have never known before.