東ソー バイオサイエンス事業部　計測分野 Official site

In the purification process of antibody drugs, chromatography purification using Protein A is commonly employed. The purification with Protein A specifically binds to the Fc region of antibodies, allowing for the highly selective recovery of the target antibody even from raw materials with many impurities, such as culture supernatants, making it easier to achieve high purity. In this video, we will introduce the features of our alkaline-resistant Protein A affinity packing material "Super A" in comparison with products from other companies.

In the purification process of antibody drugs, chromatography purification using Protein A is commonly employed. The purification with Protein A specifically binds to the Fc region of antibodies, allowing for the high-selective recovery of the target antibody from raw materials with many impurities, such as culture supernatants, making it easier to achieve high purity. In this video, we will introduce the features of our alkaline-resistant Protein A affinity filler "Super A" in comparison with other products.

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During the production of bispecific antibodies (BsAb), various impurities such as mispaired antibodies, aggregates, incomplete antibodies, and fragment antibodies are often generated. Therefore, in the downstream purification process of BsAb, it is important to effectively separate and remove these impurities derived from various products and process-related impurities using chromatography. This report presents the separation modes of chromatography used in the isolation and purification of BsAb, along with the corresponding packing materials and separation conditions.

Anion exchange chromatography (AEC) is often used for the separation and purification of oligonucleotides. However, modified oligonucleotide complexes tend to be highly hydrophobic, which can lead to the formation of aggregates and higher-order structures, resulting in poor separation and decreased recovery rates. Numerous reports have been made on solutions to this issue, including investigations into separation conditions such as elution buffer composition. This summary compiles information on the elution buffers used in chromatography separations, such as AEC separation of modified oligonucleotide complexes.