Technical Data: Separation of Stereoisomers of Plasmid DNA pBR322
Introducing a method for the separation of stereoisomers of plasmid DNA pBR322 using anion exchange chromatography!
In general, plasmid DNA is obtained through purification from E. coli culture broth. The culture broth contains not only contaminating components such as host-derived genomic DNA, RNA, and proteins, but also open circular DNA (Open circular) and linear DNA, which are structural isomers of the target plasmid. Since these can reduce the efficiency of gene expression, clinical plasmid DNA is required to have a purity of over 90% for supercoiled DNA. Therefore, we examined the separation of structural isomers of plasmid DNA pBR322 using anion exchange chromatography, and we would like to introduce this study. *For more details, please refer to the PDF document or feel free to contact us.*
basic information
For more details, please refer to the PDF document or feel free to contact us.
Price range
P1
Delivery Time
OTHER
Applications/Examples of results
For more details, please refer to the PDF document or feel free to contact us.
