【Technical Data】UHPL-SEC column with reduced sample adsorption!
The UHPL-SEC column that suppresses sample adsorption is now decided! This is a must-see for everyone struggling with initial adsorption in SEC columns.
This is a technical document explaining the features of the new TOSOH SEC column "TSKgel UP-SW3000-LS," which addresses the phenomenon of non-specific adsorption of proteins (initial adsorption) often observed when equilibrating a new size exclusion chromatography (SEC) column with elution buffer and injecting protein samples. This phenomenon is particularly prevalent with antibody samples, where aggregates or dimers may adsorb, leading to a reduction in elution peaks or, in some cases, no peaks being eluted at all. Such occurrences are seen in nearly all SEC columns.
basic information
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Applications/Examples of results
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