Technical data: The AEC separation of modified oligonucleotide complexes is now settled!
Improvement of separation using an anion exchange resin TSKgel SuperQ-5PW(20) and points regarding the elution solution.
Anion exchange chromatography (AEC) is commonly used for the separation and purification of oligonucleotides. However, modified oligonucleotide complexes tend to be highly hydrophobic, which can lead to the formation of aggregates and higher-order structures, resulting in poor separation and decreased recovery rates. Numerous reports have been published on solutions to this issue, including investigations into separation conditions such as elution buffer composition. This document summarizes the elution buffers used in chromatography separations, such as AEC separation of modified oligonucleotide complexes.
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- AEC separation using TSKgel SuperQ-5PW(20) (followed by separation with RPC) - AEC analysis of drug-antibody conjugate ratios (DAR=0, 1, 2) - SEC analysis using TSKgel UP-SW2000; separation of sense, antisense, and double-stranded - AEC separation using weak anion exchange resin (DEAE type)
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The BioScience Division is engaged in business in the "Measurement Field," which includes liquid chromatography, columns, and separation agents, and the "Diagnostics Field," which focuses on immunodiagnostics, glycohemoglobin analysis, and genetic testing. In each field, we are expanding our product lineup based on distinctive technologies and establishing sales bases not only in Japan but also overseas for global business development. Additionally, the BioScience Division consistently handles everything from the development, manufacturing, and sales of equipment and reagents to maintenance and customer support, all managed by the Tosoh Group, including its affiliated companies.