[HPLC Case Study] Analysis of Dairy Beverages by Automatic Derivatization of Amino Acids
High-precision and convenient automatic quantitative analysis of amino acids using the custom injection program function of an autosampler.
Many amino acids do not absorb ultraviolet (UV) light, making it difficult to improve sensitivity and selectivity with conventional analytical methods, which are also complicated and lack stability. This technical document introduces a case where a custom injection program feature in the auto-sampler of the Thermo Scientific Vanquish Core HPLC system was utilized to derivatize 20 types of amino acids simultaneously using a pre-column derivatization method for simultaneous analysis. This system enables highly accurate quantitative analysis as it is not affected by the degradation after the derivatization reaction. Validation using commercially available beverages showed that selective quantification was possible even in complex sample matrices such as food. 【Features】 ■ Simultaneous derivatization and analysis of 20 types of amino acids ■ High-precision quantitative analysis achieved through pre-column derivatization ■ Provides good linearity and high reproducibility ■ Selective detection even in complex sample matrices ■ Automation by the auto-sampler avoids human error and improves work efficiency
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[Device Configuration] Vanquish Core HPLC System Pump: Thermo Scientific Vanquish Quaternary Pump C Autosampler: Thermo Scientific Vanquish Split Sampler CT Column Oven: Thermo Scientific Vanquish Column Compartment C Detector: Thermo Scientific Vanquish Fluorescence Detector F Software: Thermo Scientific Chromeleon Chromatography Data System 7.3
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