Genovis AB FabRICATOR (IdeS)
Proteases useful for the analysis and evaluation of antibody drugs and ADCs (antibody-drug conjugates) including biosimilars.
FabRICATOR (IdeS) is a unique enzyme that digests specific sites just below the hinge region of antibodies. It generates a uniform pool of F(ab')2 and Fc/2 with a 30-minute incubation. No substrates other than IgG are known. Typically, IgG is digested within 30 minutes, but there is no risk of excessive digestion even with longer incubation times. FabRICATOR digests IgG under physiological conditions, maintaining its immunoreactivity. The enzyme activity is maximized at 37°C, but it can also be used at room temperature by extending the reaction time. FabRICATOR digests all subclasses of IgG from humans, monkeys, rabbits, and sheep. However, its activity against mouse IgG2a and IgG3 is limited. For the digestion of these antibodies, the use of FabRICATORZ is recommended. ● Genovis actively holds web meetings and seminars. Please feel free to contact us for adjustments according to your requests.
basic information
FabRICATOR is cloned from Streptococcus pyogenes and expressed in Escherichia coli. This enzyme contains a His tag and has a molecular weight of 37,725 Da. FabRICATOR is a recombinant modified enzyme of Ides developed by Genovis. <Main Product Lineup> FabRICATOR Enzyme Lyophilized product. Available in 8 x 100 units, 96 x 100 units, 2000 units, 5000 units, and 5 x 5000 units. FragIT - Immobilized FabRICATOR Spin column filling product. Since FabRICATOR is immobilized on agarose beads, it can highly prevent contamination from FabRICATOR and obtain the desired preparation. <Other Products> FabRICATOR LE (Low Endotoxin) FabRICATOR Validation Kit FragIT kit Anti-FabRICATOR Antibody FabRICATOR-HPLC
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Applications/Examples of results
Subunit analysis of antibody drugs including biosimilars Characterization of antibody-drug conjugates (ADCs) Glycan analysis of the Fc domain of IgG antibodies
