Anion exchange chromatography (AEC) is often used for the separation and purification of oligonucleotides. However, modified oligonucleotide complexes tend to be highly hydrophobic, which can lead to the formation of aggregates and higher-order structures, resulting in poor separation and decreased recovery rates. Numerous reports have been made on solutions to this issue, including investigations into separation conditions such as elution buffer composition. This summary compiles information on the elution buffers used in chromatography separations, such as AEC separation of modified oligonucleotide complexes.

In 2025, Tosoh will newly install a manufacturing plant for separation and purification agents at the Nanyo Plant (Shunan City, Yamaguchi Prefecture), further enhancing production capacity. To encourage more people to use TOYOPEARL products, we will be conducting a half-price campaign. We hope you take this opportunity to discover the appeal of TOYOPEARL products and find them useful for your separation and purification needs.

We have published a data collection of tests and analyses of herbal medicines using TSKgel. This data collection introduces analysis examples for 20 items among the herbal medicines listed in the eighteenth revised edition of the Japanese Pharmacopoeia. 【Analysis subjects included】 1. … Onsei-in extract 2. … Ougon 3. … Kouren-ketokutou extract 4. … Kakkontou extract 5. … Kamikihitou extract 6. … Kanzo 7. … Keishibukuryougan extract 8. … Kouboku 9. … Gojyuuyutou extract 10. … Goleisan extract 11. … Saiko 12. … Saiko-keishitou extract 13. … Sanshishi 14. … Shakuyaku 15. … Ninjin 16. … Hanjaku-houbakutou extract 17. … Byakko-ka-ninjin-tou extract 18. … Botanki 19. … Rokkunzitou extract 20. … Reikeijutsugantou extract * A printed version is also available. If you would like one, we can send it by mail. Please contact us via "Inquiries" to request this.

We have published SEC (GPC/GFC) data for various synthetic polymers! Introducing GPC data for 112 actual samples! If you are having trouble with molecular weight distribution measurement of polymers or GPC measurement, please take a look! *We also have a printed version available. If you would like one, we can send it to you by mail. Please contact us through "Inquiries" to let us know.

In general, plasmid DNA is obtained through purification from E. coli culture broth. The culture broth contains not only host-derived genomic DNA, RNA, and proteins, which are impurities, but also open circular DNA (Open circular) and linear DNA, which are structural isomers of the target plasmid. These can reduce the efficiency of gene expression, so clinical plasmid DNA is required to have a purity of over 90% for supercoiled DNA. Therefore, in this study, we investigated the separation of structural isomers of plasmid DNA pBR322 using anion exchange chromatography, and we would like to introduce it.