We have released technical documentation on the AEC separation of modified oligonucleotide complexes.
東ソー バイオサイエンス事業部 計測分野
Anion exchange chromatography (AEC) is often used for the separation and purification of oligonucleotides. However, modified oligonucleotide complexes tend to be highly hydrophobic, which can lead to the formation of aggregates and higher-order structures, resulting in poor separation and decreased recovery rates. Numerous reports have been made on solutions to this issue, including investigations into separation conditions such as elution buffer composition. This summary compiles information on the elution buffers used in chromatography separations, such as AEC separation of modified oligonucleotide complexes.
