What is mass spectrometry? An explanation of the types and principles of ionization methods, as well as considerations for LC conditions.

This article discusses the issue of ionic substances not being retained in the analysis of reverse phase systems, along with the causes and methods for improvement (ion suppression chromatography and ion-pair chromatography).

An explanation of the causes of tailing of ionic substances in the analysis of reverse phase systems and the selection of mobile phases for improvement.

A clear explanation of the key points of column technology and system design to maximize the performance of particles with a diameter of 2 μm or less, achieving the separation and speed predicted by theory (UPLC separation).

"Smart MS" BioAccord System Latest Information Webinar We will hold a WEB seminar (on-demand) introducing the product overview of the BioAccord LC-MS system, which supports routine analysis of biopharmaceuticals, peptide drugs, and nucleic acid drugs. Additionally, we will explain the Multi Attribute Method (MAM), analysis of nucleic acid drugs, and new applications for adeno-associated viruses (AAV).

From the perspective of retaining polar compounds, we will introduce the utilization of reverse phase and HILIC modes in the food and environmental fields, along with key points for selecting useful columns.

We will introduce how to deal with a carryover situation and how to identify the causes and solutions when a ghost peak is observed.

Introducing an information processing tool that supports HDMS(E) data and effectively utilizes the advantages of maximized peak capacity through ion mobility separation in a powerful and efficient new workflow.

An overview of GPC (SEC/GFC) used in polymer analysis and key points for basic analysis will be explained.

A webinar discussing the necessary considerations, issues, and solutions in the development of analytical methods for ion exchange and size exclusion chromatography. The content is applicable not only to biopharmaceuticals but also to the analysis of other proteins.

This discusses the issues revealed by the results of a blind test for protein identification conducted by multiple facilities, and what is necessary to achieve comprehensive and reliable identification.

The key to solving problems lies in identifying the cause. This explains methods for quickly and reliably finding the cause, focusing on solid-phase extraction.

Introducing the efficiency of reverse phase analysis of intact proteins, as well as the issues of quantitative analysis using reverse phase and the methods for developing analytical techniques.

Introducing UPLC and columns specifically for glycan separation, multifunctional mass spectrometers, and glycan analysis using informatics.

This is an easy-to-understand introduction for those who need sample preprocessing, explaining its necessity and how to choose the most suitable preprocessing method for their analysis.

The molecular weight distribution and additives of polymer materials are significant factors that greatly contribute to the physical properties and lifespan of the materials, making them widely analyzed from material development to quality control. Typically, these two analyses employ different separation modes, requiring dedicated systems and separate pretreatment methods. However, efficiency can be improved by conducting both analyses in a single analysis using the same pretreatment sample. In this webinar, you will learn about the new technology of GPC/MS and the application of simultaneous measurement of molecular weight distribution and additive quantification. Main content of the webinar: ■ Principles and advantages of high-resolution GPC ■ How to utilize GPC + RI detector + MS detector ■ Simultaneous analysis methods and approaches for molecular weight distribution and additive quantification Target audience: ■ Those using GPC systems ■ Those using LC, LC/MS, and GC/MS for additive analysis ■ Individuals involved in research and development, quality assurance, and management of polymer materials Program: ■ Measurement of molecular weight distribution and additive quantification in polymer materials ■ Principles and characteristics of high-resolution GPC ■ Challenges in GPC/MS systems and analysis ■ Case studies of simultaneous analysis of molecular weight distribution and additives

Please explain what chromatography is, what types of stationary and mobile phases are used in liquid chromatography (LC), and what kinds of separation modes exist in LC.

CPhI Webinars: "COVID-19" Vaccine & Therapeutics Research and Development Webinar Waters held an LC/MS Webinar related to the novel coronavirus at CPhI Webinars 2020 (organized by Informa Markets Japan Co., Ltd.). Thank you to everyone who participated. The on-demand version is now available, so please take a look. [Title] "Latest LC/MS Technology Information Accelerating Novel Coronavirus Research" [Abstract] Among the analytical techniques supporting research on therapeutics and vaccine development for COVID-19, liquid chromatography-mass spectrometry (LC/MS) is at the core of separation analysis. Here, we will introduce the latest analytical techniques and case studies targeting proteins, lipids, glycans, nucleic acids, and more.

When assessing the safety and efficacy of pharmaceuticals, the quality and robustness of analytical data are extremely important. The currently discussed ICH guidelines are considering a lifecycle approach to improve the consistency and robustness of analytical method quality over the long term. By deploying the developed analytical methods to quality control labs using the latest HPLC, it is possible to reduce OOS (Out of Specification) and OOT (Out of Trend) occurrences. In this webinar, we will explain the challenges of routine analysis in quality control labs and their solutions. We will also introduce a new product, Arc HPLC, which allows for a smooth transfer of existing LC analytical methods and enables the acquisition of higher quality data.

Compounds or their metabolites found in pharmaceuticals, cosmetics, and food can have high hydrophilicity. In environmental monitoring, hydrophilic and highly polar compounds may also be targeted in water system environmental analysis. Since these compounds are difficult to analyze using conventional reverse phase chromatography, hydrophilic interaction chromatography (HILIC) is often suitable. On the other hand, many people may think, "It sounds complicated just by the name," and have not yet tried HILIC, or they may not be satisfied with their current HILIC analysis. In this seminar, we will introduce the principles of HILIC analysis, key points for method development, and columns suitable for HILIC analysis. Program: ■ Basics of HILIC mode ■ Development of HILIC analytical methods ■ Columns suitable for HILIC mode and analysis examples Target Audience: ■ Those analyzing highly polar and hydrophilic compounds ■ Those planning to conduct analyses in the future ■ Those considering improvements to their current HILIC analysis

In this live webinar, we will have a presentation from Shionogi Pharma on the high-sensitivity analysis of nitrosamine impurities. ■Target Audience Those developing impurity analysis methods and those involved in quality control analysis.

A clear explanation with examples on how to verify whether the LC system is performing at its intended capacity.

Have you ever struggled with scaling up from analytical to preparative chromatography? Many people may be facing issues such as the chromatogram examined in the analysis not being reproducible, excessive solvent consumption, or a short lifespan of the preparative column. In this seminar, we will explain tips for preparative purification to maximize the load and obtain as many target substances as possible in a single purification, using analytical examples. Program: ■ Workflow of preparative purification ■ How to determine preparative conditions ■ Tips for selecting preparative columns ■ Introduction of examples of preparative purification

A clear explanation of the QuEChERS method suitable for simultaneous analysis.

An explanation with examples of technology that can streamline and simplify the entire process from extraction to measurement in analysis, based on the fundamentals of two-dimensional LC.

Introducing the disulfide bond mapping feature added to BiopharmaLynx.

Introducing the application of UPLC/Q-Tof MS systems and bioinformatics to routine analysis of the characteristics of antibodies and protein pharmaceuticals.

It's just a vial. Yet, it's a vial. The vial is the component that comes into contact with the sample solution for the longest time. This explains how to choose vials suitable for troubleshooting and analytical techniques derived from vials.

Introduction of actual application examples regarding column selection for retaining highly polar compounds in reversed-phase chromatography.

Introducing the performance of UltraPerformance Convergence Chromatography (UPC2), which achieves ultra-fast, robust, and highly reproducible analysis, along with examples of chiral separation applications.