"Next-Generation CHO Cell Line Development Service Integrating AI and Process Intensification - Innovation of Sartorius CLD Workflow" In biopharmaceutical development, establishing high-quality cell lines in a short period has become increasingly important. Sartorius's Cell Line Development (CLD) service has supported the approval of 7 antibody products and over 80 clinical projects to date. Seminar Points: ■ The latest workflow that selects optimal clones by AI analysis of nano-well images and fluorescence data immediately after single-cell cloning with CellCelector ■ A series of CLD processes that continue from Clone Evaluation, quality analysis, scale-up, to technology transfer to CDMO ■ Process Intensification strategies based on High Inoculation Fed-Batch and Perfusion culture ■ Media optimization service that can be used independently (supporting non-GMP prototypes to GMP manufacturing scale) Date and Time: December 3 (Wednesday) 12:20–13:20 Venue: Omiya Sonic City, Exhibition Hall 2

The final filtration sterilization process in biopharmaceutical manufacturing is a critical process that requires ensuring the efficacy of the product while guaranteeing safety. The PVDF filters commonly used in this process have excellent low adsorption properties, but they contain PFAS, which raises concerns about their impact on the environment and health. Sartorius has addressed this challenge by developing a new filter made from PES material that intentionally does not use PFAS, called "Sartopore Evo." This product significantly reduces protein adsorption compared to conventional PES filters due to its innovative surface treatment technology, achieving low adsorption properties comparable to PVDF filters. Additionally, the excellent liquid flow characteristics of the PES material enable high-flow filtration, contributing to the efficiency of the final filtration sterilization process. In this webinar, we will provide a detailed introduction to the performance and benefits of the new PES filter "Sartopore Evo." 【Seminar Overview】 ▪ Date: November 27 (Thursday) at 3:00 PM (approximately 30 minutes) ▪ Method: Webinar recorded broadcast [Japanese, free of charge] *We apologize, but participation from companies in the same industry and email addresses other than those of your organization is not permitted. Thank you for your understanding.

The Ambr 250 High Throughput is a high-throughput bioreactor that accelerates process development and process characterization analysis. It enables simultaneous cultivation of up to 24 samples while incorporating automated control via a robotic arm and integration with external analytical devices. The culture containers are single-use specifications of 100-250 mL and are compatible with perfusion culture and microcarrier culture. This device has been enhanced based on customer feedback and was launched as the second generation, Ambr 250 High Throughput Generation 2, starting in 2025 to demonstrate further performance as a scale-down model. In this webinar, we will introduce the device overview and the enhanced features in Generation 2. 【Seminar Overview】 ▪ Date: October 16 (Thursday) at 15:00 (approximately 30 minutes) ▪ Method: Webinar recorded broadcast [Japanese, Free] *We apologize, but participation from companies in the same industry and email addresses other than those of your organization is not permitted. Thank you for your understanding.

In this webinar, we will introduce Sartorius's innovative virus quantification device, Virus Counter Plus. Virus Counter Plus employs flow cytometry technology that allows for the direct measurement of virus particle counts in just a few hours, compared to traditional plaque assays and qPCR, enabling rapid virus quantification. The only work required for measurement is mixing the sample with the reagent, eliminating the need for skilled techniques. This prevents the task from becoming dependent on specific individuals and significantly reduces training burdens. Additionally, it is equipped with an auto-sampler, achieving high-throughput measurements. By setting up dozens of samples before leaving work, automatic analysis can proceed overnight, allowing results to be checked the following morning. This accelerates development speed, enables real-time monitoring of processes, and facilitates quick decision-making during manufacturing. 【Seminar Overview】 ▪ Date: October 16 (Thursday) from 3 PM (approximately 30 minutes) ▪ Method: Webinar recorded broadcast [Japanese, free of charge] *We apologize, but participation from companies in the same industry and email addresses other than those from your organization is not permitted. Thank you for your understanding.

We will be exhibiting and presenting a luncheon seminar at BioJapan 2025, which will be held at Pacifico Yokohama from October 8 to 10, 2025. At our booth, we will introduce our cell line development services and media optimization services that achieve a scalable fed-batch process with a monoclonal antibody titer of up to 10 g/L in just 9 weeks, from DNA to RCB. We will also showcase single-use culture vessels for process development. In addition, during the luncheon seminar, we will provide a clear overview of Sartorius' CLD services, making it easy to understand for those who are new to the topic. If you are considering shortening timelines, optimizing product quality, or exploring flexible development strategies, please join us. We look forward to seeing you there. Event Overview: October 8 (Wed) - 10 (Fri), 2025, Pacifico Yokohama Exhibition Booth: Small Booth Number D-109 Luncheon Seminar: Title: Minimizing Risks in CHO Cell Line Development through Automation and Predictive Models Seminar Date and Time: October 9 (Thu) 11:30 AM - 12:30 PM

This year, Sartorius Stedim Japan will hold webinars on a wide range of topics. You can check the 2023 schedule from the "Related Materials" section on this page. If you are unable to attend on the day of the event, you will receive a recording of each webinar after it concludes if you register. By clicking on "Related Links," you can see the webinars available for registration. We look forward to your registration. If you have any questions, please feel free to contact us.

This year, Sartorius Stedim Japan will hold webinars on a wide range of topics. You can check the schedule for 2025 on the "Related Materials" section of this page. If you are unable to participate on the day of the event, you will still receive a recording of each webinar after it concludes if you register. By clicking on the "Scheduled Webinars for 2025" link below, you can see the webinars available for registration. We look forward to your registration. If you have any questions, please feel free to contact us.

This year, Sartorius Steadycam Japan will hold webinars on a wide range of topics. You can check the 2024 schedule from the "Related Materials" section on this page. If you are unable to attend on the day of the event, you will receive a recording of each webinar after it concludes if you register. By clicking on "Related Links," you can see the webinars available for registration. We look forward to your registration. If you have any questions, please feel free to contact us.

Participation in this webinar has ended. If you wish to view it, please contact us. We will send you the URL for the recorded video at a later date. Sartorius has developed CHO cell lines for over 300 projects, with 7 projects approved and more than 80 in clinical trials. The projects include IgG1, 2, 4, multi-specific antibodies, Fc fusion proteins, enzymes, hormones, new drugs, and biosimilars. Our customers range from large companies to startups, with over 70% of them being repeat clients. In this webinar, we will introduce the details of our flexible and comprehensive services, including CHO cell line development, MCB/WCB manufacturing and testing, molecular characterization, FUT8 Knock Out, provision of optimized media, and process enhancement. *We sincerely apologize, but we kindly ask that participants refrain from joining with email addresses other than those from your company or organization. Thank you for your understanding.

Participation in this webinar has ended. If you wish to view it, please contact us. We will send you the URL for the recorded video at a later date. In recent years, the use of single-use products in biopharmaceutical manufacturing has become common, but the issue of "foreign substances" has become a significant concern. We believe that not only our products but also others face similar issues, and currently, there are no solutions to address this. The contamination control strategy (CCS) is frequently emphasized in the PIC/S Annex 1, and it is crucial to understand what measures are being taken to safely use single-use products. This time, we aim to provide the latest information on these initiatives and hope to gain your understanding and feedback. *We sincerely apologize, but we ask that individuals from competing companies and those using email addresses other than those from their affiliated organizations refrain from participating. Thank you for your understanding.

We will be exhibiting and presenting a seminar at TIDES Asia 2025, which will be held in Kyoto from February 26 to 28, 2025. TIDES Asia is the leading conference in Asia that gathers experts in oligonucleotides, peptides, mRNA, and genome editing therapeutics. At this conference, we plan to showcase high-throughput TFF equipment for parallel screening and hold a seminar on innovative solutions for peptide therapeutic manufacturing. We look forward to your visit. 【Event Overview】 Date: February 26 (Wednesday) - 27 (Friday), 2025 Location: The Westin Miyako Hotel Kyoto *Pre-registration required 【Our Seminar】 Title: Fit for Future: Advancing Bioprocess Manufacturing by Identifying Top Performing Clones and Enhancing Productivity through Process Intensification Seminar Date and Time: 27th (Thursday) at 15:45

To maximize the performance of CHO cells, it is necessary to select the optimal medium for each clone and further optimize the process parameters. Sartorius offers a variety of basic media and feeds suitable for Fed-batch culture of major CHO cell lines such as CHO-DG44, K1, S, and GS. In this webinar, we will introduce a case study that combines these CHO media, the automated microbioreactor Ambr15, and the experimental design software MODDE to conduct medium screening and process optimization in a short period. We will also provide information about our Benchmark Study service, which allows you to outsource the selection of the optimal medium and process development for your specific cell line to our research institute in Germany. 【Seminar Overview】 ▪ Date: March 6, 2025 (Thursday) from 3:00 PM (approximately 40 minutes) ▪ Method: Webinar recorded broadcast [Japanese, Free] If you are unable to participate on the day, please register first. We will send you a viewing URL later. *We kindly ask that participants from other companies in the same industry, as well as those using email addresses not affiliated with your organization, refrain from joining. Thank you for your understanding.

In the pharmaceutical and biopharmaceutical industry, there is an increasing need to maintain world-class quality and adopt the latest technologies to enhance technical skills and productivity. The biggest challenge faced is responding to new compliance measures and regulations. At Sartorius, we have prepared training courses focused on practical exercises, aimed at enabling our customers to perform their work safely and efficiently. The courses listed can also be customized to meet your specific needs. If necessary, they can be held remotely. You can apply using the "Dedicated Form" on page 11 of the "Related Materials" or through the "Related Links." You can check the schedule from the "Related Materials." We look forward to your participation in Sartorius training.

Participation in this webinar has ended. If you wish to view it, please contact us. We will send you the URL for the recorded video at a later date. There is significant interest in improving the productivity of downstream processes, as it is possible to reduce product costs by enhancing the purification process of downstream processes. In this webinar, we will introduce the features of Sartobind Rapid A, which eliminates the need for affinity resin and achieves a reduction in process time. *We apologize, but participation from other companies in the same industry, as well as from email addresses not associated with your organization, is not permitted. Thank you for your understanding.

Participation in this webinar has ended. If you wish to view it, please contact us. We will send you the URL for the recorded video at a later date. Traditionally, the use of lentiviruses in prominent CAR-T production and the application of electroporation in academia have been common in gene introduction into blood cells. Our series of cationic lipid-based mRNA introduction reagents, which contain IM21.7c as a raw material, have achieved gene introduction into various blood cell types that overturn these conventional norms. In this webinar, we hope to share these results to serve as a reference for the future development of gene introduction T cells. *We sincerely apologize, but participation from companies in the same industry and from email addresses other than those of your affiliated organization is not permitted. Thank you for your understanding.

This event has ended. We will be exhibiting and presenting at BioProcess International Asia 2024, which will be held in Kyoto from October 21 to 23, 2024. We will introduce Sartorius's cell line development solutions that comprehensively support the commercialization of biopharmaceuticals through products, services, experience, and expertise. 【Event Overview】 Date: October 21 (Monday) 9:00 AM - October 23 (Wednesday) 5:30 PM Location: The Westin Miyako Kyoto *Pre-registration required 【Our Seminar】 Title: Fit for Future: Advancing Bioprocess Manufacturing by Identifying Top Performing Clones and Enhancing Productivity through Process Intensification Seminar Date: October 22 (Tuesday) 9:30 AM

This event has ended. We will be exhibiting and presenting at the 31st Annual Meeting of the Japan PDA Pharmaceutical Science Society, which will be held at Tower Hall Funabori on December 5th and 6th, 2024. In the luncheon seminar, we will introduce quality assurance for single-use products regarding foreign substances and our efforts towards sustainability. At our exhibition booth, we will showcase single-use and filter products, as well as validation services. Please stop by. 【Event Overview】 Date: December 5th (Thursday) - 6th (Friday), 2024 Location: Tower Hall Funabori, 4-1-1 Funabori, Edogawa-ku, Tokyo 134-0091 *Pre-registration required 【Our Seminar】 Title: Efficiency of Single-Use Products and Quality Assurance of Flexsafe Bags (Leak, E|L, Management of Foreign Substances) Seminar Date and Time: December 5th (Thursday) 12:45-13:45

Participation in this webinar has ended. If you wish to view it, please contact us. We will send you the URL for the recorded video at a later date. The media required for pharmaceutical development varies by development phase, making it important to choose a media partner with flexible options and a robust supply system. This webinar will focus on CHO media, introducing services for developing media with optimal compositions for your cell lines, prototype manufacturing services for supplying specified compositions in a short period, and a global manufacturing system that supports commercial production. 【Seminar Overview】 ▪ Date: September 5 (Thursday) from 15:00 (approximately 30 minutes) ▪ Method: Webinar recorded broadcast [Japanese, free] If you are unable to participate on the day, please register first. We will send you the URL for viewing later. *We regret to inform you that participation from other companies in the same industry and email addresses not associated with your organization is not permitted. Thank you for your understanding.

This event has concluded. We will be exhibiting and presenting at the Cell Culture Medium Symposium at Shonan, held at Shonan Ai Park on September 13, 2024 (Friday). At our booth, we will introduce Sartorius's wide range of media, raw materials, and services that meet the needs of cell culture. *You can view the program in the "Related Materials." [Event Overview] Date and Time: September 13, 2024 (Friday) 10:00 AM - 5:45 PM Location: Shonan Ai Park or online *Pre-registration required [Our Seminar] Title: Recombumin | Enabling safe and robust manufacturing across various modalities with recombinant human albumin Speaker: Shiori Akiyama (Sales Development Specialist - Cell Lines, Media, Various Tests) Seminar Time: 3:40 PM - 4:10 PM Overview: This seminar will focus on Recombumin, a recombinant human albumin that improves cell viability after cryopreservation and has an effect on suppressing the aggregation of cells and vectors.

Participation in this webinar has ended. If you wish to view it, please contact us. We will send you the URL for the recorded video at a later date. The field of cell therapy and regenerative medicine is a new area that can treat diseases for which there have been no effective treatments until now. Additionally, it is expected to grow globally as an industry and has been gaining importance. In this field, it is a crucial step to recover cultured cells without damaging them and in a sterile manner. Ksep makes this step possible, which has been difficult with conventional methods. We will explain why this is possible with Ksep, comparing it to traditional methods. Furthermore, Ksep is also expected to be utilized in the field of antibody therapy. We will also explain the benefits in this area. This webinar will focus on the Ksep 50, which will be launched in 2024, and introduce the above content. Participation in this webinar has ended. If you wish to view it, please contact us. We will send you the URL for the recorded video at a later date.

Registration for this webinar has ended. If you wish to view it, please contact us. We will send you the URL for the recorded video at a later date. Contamination Control Strategy (CCS) is a key focus in biopharmaceutical manufacturing, with one aspect being aseptic sampling. Process management is influenced by the quality of the samples taken, so appropriate sampling methods and containers for each process are crucial. In this webinar, we will not only introduce aseptic sampling devices but also present the most suitable sampling methods and containers based on whether the focus is on confirming sterility or process management. [Seminar Overview] ▪ Date: April 4 (Thursday) at 3:00 PM (approximately 40 minutes) ▪ Format: Webinar recorded broadcast [Japanese, Free] *We sincerely apologize, but participation from companies in the same industry and from email addresses other than those of your organization is not permitted. Thank you for your understanding.

Registration for this webinar has closed. If you wish to view it, please contact us. We will send you the URL for the recorded video at a later date. In biopharmaceutical manufacturing, productivity, consistency, and safety of processes are emphasized, but it is said that ensuring stability in cell gene therapy is particularly challenging. Recombumin, due to its diverse physicochemical properties, improves cell survival rates after freeze preservation and suppresses aggregation of cells and vectors, thereby enhancing process productivity. Its strengths include very small inter-batch variability, high safety due to the absence of animal-derived components, and a regulatory compliance system in place in Japan. In this webinar, we will welcome Mr. Kazushige Sugama, a technical advisor from Albumedix, as a guest speaker to introduce examples of pharmaceutical manufacturing utilizing Recombumin's properties, particularly in gene cell therapy. 【Seminar Overview】 ▪ Date: March 7 (Thursday) from 3:00 PM (approximately 45 minutes) ▪ Format: Webinar recorded broadcast [Japanese, Free] *We sincerely apologize, but participation from companies in the same industry and email addresses other than those of your organization is not permitted. Thank you for your understanding.

Participation in this webinar has ended. If you wish to view it, please contact us. We will send you the URL for the recorded video at a later date. The revision of the EU GMP guidelines Annex 1 aims to deepen the relationship between Sartorius and our customers through mutual understanding of Quality Risk Management (QRM) and Contamination Control Strategy (CCS), while following its content. This time, we will particularly focus on the filling process and provide solutions using our products. [Seminar Overview] ▪ Date: February 8 (Thursday) at 15:00 (approximately 30 minutes) ▪ Method: Webinar recorded broadcast [Japanese, free of charge] *We kindly ask that participants refrain from joining with email addresses other than those from your own company or organization. Thank you for your understanding.

Participation in this webinar has ended. If you wish to view it, please contact us. We will send you the URL for the recorded video at a later date. We will introduce the content of the revised EU GMP Guidelines Annex 1. While reviewing this content, we aim to deepen the relationship between Sartorius and our customers through mutual understanding, particularly regarding Quality Risk Management (QRM) and Contamination Control Strategy (CCS). Additionally, we will provide information on Sartorius's sterilization validation and the latest information on Extractables and Leachables (E|L), which we hope you will find useful. [Seminar Overview] ▪ Date: December 7 (Thursday) at 3:00 PM (approximately 30 minutes) ▪ Format: Webinar recorded broadcast [Japanese, Free] *We sincerely apologize, but participation from companies in the same industry and email addresses other than those of your organization is not permitted. Thank you for your understanding.

In the pharmaceutical and biopharmaceutical industry, there is an increasing need to maintain world-class quality and adopt the latest technologies to enhance technical skills and productivity. The biggest challenge faced is responding to new compliance measures and regulations. At Sartorius, we have prepared training courses focused on practical exercises, aimed at enabling our customers to perform their work safely and efficiently. The courses listed can also be customized to meet your specific needs. If necessary, they can be conducted remotely. You can apply using the "Dedicated Form" on page 11 of the "Related Materials" or through the "Related Links." You can check the schedule from the "Related Materials." We look forward to your participation in Sartorius training.

Participation in this webinar has ended. If you wish to view it, please contact us. We will send you the URL for the recorded video at a later date. We will consider what we have learned from the Covid-19 pandemic and how to apply it in the future. To prepare for the next pandemic, which may occur at any time, we recommend a flexible Ballroom concept. This concept reduces the number of classes between rooms in manufacturing facilities and helps to control HVAC costs. At the same time, it also facilitates space-saving and allows for rapid scalability. *This webinar is an extended version of the content presented in our technical seminar at BIopharma EXPO 2023. [Seminar Overview] ▪ Date: November 16 (Thursday) from 3:00 PM (approximately 30 minutes) ▪ Format: Webinar recorded broadcast [Japanese, free of charge] *We sincerely apologize, but participation from companies in the same industry and email addresses other than those of your organization is not permitted. Thank you for your understanding.

In the pharmaceutical and biopharmaceutical industry, there is an increasing need to maintain world-class quality and adopt the latest technologies to enhance technical skills and productivity. The biggest challenge faced is responding to new compliance measures and regulations. At Sartorius, we have prepared training courses focused on practical exercises with the aim of enabling our customers to perform their work safely and efficiently. The courses listed can also be customized to meet your specific needs. They can be held remotely if necessary. You can apply using the "Dedicated Form" on page 10 of the "Related Materials" or through the "Related Links." You can check the schedule from the "Related Materials." We look forward to your participation in Sartorius training.

Participation in this webinar has ended. If you wish to view it, please contact us. We will send you the URL for the recorded video at a later date. Adeno-associated virus (AAV) vectors are gaining attention for their excellent suitability as vectors in gene therapy. The production of AAV vectors commonly uses transient expression system HEK cells, but the examination of their culture and production conditions is complex and time-consuming. Additionally, there is no standardized process, and product-specific characterization and safety testing are required. In this webinar, we will outline the manufacturing and process development of AAV vectors, followed by an introduction to Sartorius' culture-related solutions, including HEK media that simplify media selection, comprehensive characterization covering key evaluation items, and cell bank production. [Seminar Overview] ▪ Date: September 28 (Thursday) from 3:00 PM (approximately 45 minutes) ▪ Format: Webinar recorded broadcast [Japanese, free of charge] *We apologize, but participation from companies in the same industry and email addresses other than those of your organization is not permitted. Thank you for your understanding.

Participation in this webinar has ended. If you wish to view it, please contact us. We will send you the URL for the recorded video at a later date. Viral contamination is a common challenge in the manufacturing of biopharmaceuticals. In this webinar, we will introduce virus removal-related products and validation services (Confidence Virus Clearance Services) centered around the filters that Sartorius can provide. 【Seminar Overview】 ▪ Date: September 21 (Thursday) from 3:00 PM (approximately 30 minutes) ▪ Format: Webinar recorded broadcast [Japanese, Free] *We sincerely apologize, but participation from other companies in the same industry, as well as from email addresses not affiliated with your organization, is not permitted. Thank you for your understanding.

Expectations for regenerative medicine using stem cells such as mesenchymal stem cells (MSC) and induced pluripotent stem cells (iPSC) are rising, but for industrialization, there is a need for technology to produce high-quality cells in large quantities and in a stable manner. In this seminar, we will introduce xenofree stem cell culture media that do not contain components derived from animals other than humans, as well as examples of 3D culture using our microcarriers, bioreactors, and experimental design software. We will also present HEK media used for immune cell culture media and lentivirus production as products for cell therapy, such as CAR-T. 【Seminar Overview】 ▪ Date: October 12 (Thursday) from 3:00 PM (approximately 30 minutes) ▪ Format: Webinar recorded broadcast [Japanese, Free] If you are unable to attend on the day, please register first. Later, we will send you a link to download the recorded video and related product materials. *We apologize, but participation from other companies in the same industry, as well as email addresses not associated with your organization, is not permitted. Thank you for your understanding.