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Multiple SNP analyses using mass spectrometry.

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Multiple SNP analyses using mass spectrometry.

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The mass array method allows for the simultaneous analysis of multiple SNPs (single nucleotide polymorphisms).

This method is a gene analysis technique that utilizes mass spectrometry with MALDI-TOF-MS, allowing for the analysis of multiple SNP sites in a single measurement. Up to 40 SNPs can be measured simultaneously across 190 samples. The overall analysis follows the flow outlined below. Primers designed to amplify the target SNP regions without overlapping mass peaks, along with primers designed to extend only one base at each SNP site, are used to create mass differences in the DNA fragments, from which the bases are determined based on the detected mass peaks.

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Analysis of biotechnology, pharmaceuticals, cosmetics, food, and the environment.

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Multiple SNP analysis using mass spectrometry_B0295

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