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[Analysis Case] Simultaneous Analysis of 17 Free Amino Acids

C0341.jpg
OPA法の装置基本構成.png
クロマトグラフ.png

[Analysis Case] Simultaneous Analysis of 17 Free Amino Acids

C0341.jpg
C0341.jpg OPA法の装置基本構成.png クロマトグラフ.png

High-sensitivity and selective amino acid analysis is possible using the OPA post-column method.

The OPA post-column method is a technique that reacts amino acids with the fluorescent reagent OPA after separation in a column and detects the fluorescence (Figures 1, 2). This method allows for high-sensitivity analysis compared to the ninhydrin method. The fluorescent reagent OPA selectively reacts with primary amines, making it less susceptible to interference from contaminants and enabling highly selective analysis. Proline is a secondary amino acid, but it can be converted into a primary amine by adding sodium hypochlorite to the reaction solution, allowing for measurement. This document presents a case study of simultaneous measurement of 17 amino acid components, including proline (Figure 3).

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[Analysis Case] Simultaneous Analysis of 17 Free Amino Acids_C0341

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