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[Analysis Case] LC/MS/MS Analysis of Vitamin C

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[Analysis Case] LC/MS/MS Analysis of Vitamin C

Simultaneous analysis of D, L-ascorbic acid and dehydroascorbic acid.

The vitamin C contained in food refers to the total amount of reduced L-ascorbic acid and oxidized dehydroascorbic acid. Its applications include not only nutritional enhancement but also use as an antioxidant. When measuring vitamin C using HPLC, it is common to convert the oxidized form after noting that dehydroascorbic acid has no UV absorption, and then derivatize it for measurement. By quantifying dehydroascorbic acid using LC/MS/MS, it becomes possible to simplify the pretreatment and analyze with high sensitivity.

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Measurement method: LC/MS Product field: Food Analysis purpose: Product investigation, composition evaluation, identification

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[Analysis Case] LC/MS/MS Analysis of Vitamin C_C0343

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