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[LC/MS] Liquid Chromatography Mass Spectrometry

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[LC/MS] Liquid Chromatography Mass Spectrometry

It is an analytical method that detects ions with a detector and performs qualitative and quantitative analysis.

Liquid chromatography (LC) is classified as a type of chromatographic method that separates components of a liquid sample based on chromatographic principles. The detection of the separated components using a mass spectrometer is referred to as liquid chromatography-mass spectrometry (LC/MS). LC/MS is an analytical technique for the qualitative and quantitative analysis of organic compounds. - Effective for analyzing components with relatively large molecular weights or relatively high polarity. - By selecting the ionization method, it can accommodate thermally unstable substances and others. - There are various ionization methods available, allowing detection using the most suitable ionization method for the component (Electrospray Ionization (ESI), Atmospheric Pressure Chemical Ionization (APCI), Atmospheric Pressure Photoionization (APPI)). - High mass resolution can be achieved using mass spectrometers that utilize the Time-of-Flight method.

Related Link - https://www.mst.or.jp/method/tabid/133/Default.asp…

basic information

■Column Separation A liquid (or dissolved) sample is introduced into the column. Each component in the sample is separated based on the differences in affinity (retention) to the stationary phase and mobile phase within the column. By applying high pressure within the column, the separation and detection capabilities of the column separation can be enhanced. ■Detector The separated components are detected by a detector (chromatograph). Qualitative analysis can be performed from the retention time of the chromatograph (horizontal axis), and quantitative analysis can be conducted from the peak area. Additionally, estimation and identification of components can be achieved through absorption spectra and mass spectra.

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Applications/Examples of results

- Component analysis of cleaning solution - Trace impurity analysis in solution - Purity evaluation of materials - Qualitative analysis of device materials - Quantitative analysis of additives in chemical solutions - Qualitative analysis of inkjet materials - Qualitative and quantitative analysis in agriculture, biochemistry, biotechnology, and biomaterials

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