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Ultramicrotome processing

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Ultramicrotome processing

This is a processing method for cutting bulk samples using a diamond knife to produce ultra-thin sections for transmission electron microscopy with a thickness of less than 100 nm.

Ultramicrotome processing is a method that uses a diamond knife to cut bulk samples and produce ultra-thin sections for transmission electron microscopy with a thickness of less than 100 nm. Unlike processing using ion beams, it allows for the production of a wide range of sections in the atmosphere. Biological materials and soft polymer materials, which are difficult to cut at room temperature, can also be made into ultra-thin sections by cryofixation.

Related Link - https://www.mst.or.jp/method/tabid/174/Default.asp…

basic information

1. Sectioning 2. Resin embedding 3. Rough trimming 4. Trimming 5. Precision trimming 6. Ultra-thin section preparation 7. Recovery 8. For TEM observation

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Applications/Examples of results

- Structural observation of biological samples (such as hair) - Evaluation of composition distribution in layered resin samples - Evaluation of orientation of microparticles in resin - Cross-sectional analysis of polymer materials and foreign substance investigation

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