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High-Performance Liquid Chromatography (HPLC)

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High-Performance Liquid Chromatography (HPLC)

High-performance liquid chromatography (HPLC) is a method that separates and detects components in a liquid by utilizing the differences in interactions between the stationary phase and the mobile phase.

- Compounds that are relatively low molecular weight and highly polar, such as organic acids, can also be analyzed. - Low polarity compounds, such as lipids, can also be analyzed. - By using the post-column method, it is possible to eliminate the effects of interfering components that could not be completely separated. - Quantification of polymers and measurement of molecular weight distribution are possible.

Related Link - https://www.mst.or.jp/method/tabid/1321/Default.as…

basic information

By selecting the separation method and detector according to the measurement target, it is possible to qualitatively and quantitatively analyze a variety of organic compounds, ranging from low molecular weight to high molecular weight and from low polarity to high polarity.

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Applications/Examples of results

- Quantitative analysis of functional components in food - Quantitative analysis of organic acids and amino acids in food and industrial products - Quantitative analysis of lipids and fatty acids - Quantitative analysis of monosaccharides and polysaccharides - Quantitative analysis of polymers - Measurement of molecular weight distribution of polymers

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