[Analysis Case] Evaluation of Skin Permeability Using Three-Dimensional Cultured Human Skin
It is possible to conduct various evaluations of cultured human skin (morphological observation, distribution assessment of components, quantitative analysis).
■Morphological Observation The morphology of the tissue can be confirmed by staining frozen sections (e.g., HE staining, Nile blue staining). ■Fluorescence Microscopy Observation If the transmitted components have fluorescence, the fluorescent components can also be observed using a fluorescence microscope. ■Mass Microscopy Observation The distribution of components can be evaluated. Temporal changes can also be confirmed with sections taken at different times (TOF-SIMS). ■Quantitative Analysis Temporal changes in the amount of fluorescent components in the skin can also be confirmed (e.g., LC/MS/MS, fluorescence detectors, etc.).
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Applications/Examples of results
This is an analysis of skin permeability evaluation.